The impact of bio-fertilizers to increase compatibility osmolytes in the alfalfa under water stress conditions

To study effect symbiosis of fungi and bacteria in resistance to water deficit stress in alfalfa an experiment in a factorial randomized complete block design has done with three replications 2010 in greenhouse of University of Mohaghegh Ardabil, Ardabil. Water deficit stress was applied at three levels of %35, %55 and %75 of field capacity. Treatments of insemination involved inoculating of seed with strain of Mycorrhiza (Glomus mosseae), Sinorhizobium meliloti, Sinorhizobium meliloti Glomus mosseae, and control (No treatment). Results showed that with increasing stress in treatments, Sodium concentration, Proline and total soluble sugars increased, while concentration of Potassium, Phosphor was reduced, leaf area and stomatal conductance. Between treatments inoculation, Potassium, Phosphorus, Proline, Soluble sugar, stomatal conductance and leaf area with the highest values (72/6, 07/5, 14/7, 05/6, 28, 66/20 to double inoculation and lowest values (13/6, 9/2, 77/4, 73/4, 15, 33/5) was awarded to control in level of stress 35% of field capacity. After double inoculation, inoculated with mycorrhizal was more than symbiosis inoculation. But Sodium with the least amount (2.53) to double inoculation and the highest amount (3.08) to control in stress levels of 35% field capacity was related. Double inoculation with increasing water absorption, Nutrients and compatible osmolytes could improve plant resistance against the deficit. Use combination of fungi and bacteria by increasing the absorption of water and nutrients, results in increased resistance to stress deficit water in alfalfa.