The effects of pre-treatment of probiotic LactoBacillus on blood indices of common carp (Cyprinus carpio) exposed to silver nano-particles

In current study, after one week of adaptation, 250 common carp (Cyprinus carpio) were fed with two levels of Lactobacillus probiotic (106, 107) for 42 days and exposed to 50% lethal concentration of silver nanoparticles at for 10 days. Treatments included a control group, a probiotic group of 106, a probiotic group of 107, a group exposed to silver nanoparticles, a group of exposed to silver nanoparticles and fed with probiotic 106, and a group exposed to silver nanoparticles and fed with probiotic 107. Results showed that there was significant differences in MCV, MCHC, white blood cell, monocyte, neutrophil, hemoglobin, red blood cells and hematocrit between the control group and the treatments (P <0.05). Probiotics had a depletion effect on hemoglobin, red blood cells, hematocrit, and an increased effect on MCV, MCHC, white blood cells, monocytes and neutrophils. Additionally, silver nanoparticles showed depletion effects on hemoglobin, red blood cells, hematocrit, white blood cell monocyte and hemoglobin (MCHC) and increased effect on MCV and neutrophil volume. Altogether, results showed that probiotic the destructive effects of nanosilver, which led to the reduction of hemoglobin in blood cells (hypocoemic normocytic). Moreover, higher levels of probiotic (treatment 6) resulted in an increase in hemoglobin of blood cells (hyperchromic urodynamics) which requires further studies in this field.