The effect of biological compounds and fungicides to combat Biscogniauxia mediterranea causal agent of "Charcoal Disease" in vitro

In this research, sampling was conducted quite randomly from the Ghorogh forest park located in Golestan province. After isolation and purification of the fungi, charcoal pathogen was detected in Caucasian oak based on the information contained in Biscogniauxia key species identification. The effect of three fungal species including Trichoderma viride, T. atroviride and T. koningii on growth rate of charcoal pathogen in vitro evaluated using two dual culture test assay and the impact of volatile substances. All three species of Trichoderma used in dual culture tests and the impact of volatile substances, have a positive impact on the growth of the fungus. Also effect of different concentrations of fungicides including Copper oxychloride, Propiconazole, Carbendazim and Metalaxyl - Mancozeb upon the causal agent of charcoal disease were investigated in vitro culture conditions. Results showed that Propiconazol at concentrations of 50-100 ppm, and Carbendazim at concentrations of 100 ppm have the highest percentage of inhibition among the concentrations of all fungicides. Metalaxyl – Mancozeb and Copper oxychloride showed no significant difference with the control means.