Effects supplementation of zinc and Vit E on antioxidant enzyme, sexual hormone and some biochemical parameters in breeder flock of Japanese Quails

A study was conducted to investigate the effects of feeding zinc (zinc oxide) and vit E (α-tocopherol acetate) on antioxidant enzyme, sexual hormone and some biochemical parameters of Japanese quails. A total of 960 Japanese quails (Coturnix coturnix japonica) 70 d of age were housed in cages and randomly allocated to 10 treatments. Each treatment comprised 4 replicates of 24 birds (sixteen females and eight males). A 5 × 2 factorial arrangement with five levels of Zn include: 0, 40, 80, 120 and 160 mg /kg of diet and two levels of dietary Vit E (0 and 40 IU/kg of diet) was used in a completely randomized block design, by using a corn- soybean meal basal diet. Water and feed were supplied on an ad libitum basis during the 10 –wk trial. Results indicated that supplemented zinc significantly increased activity superoxide dismutase and glutathione peroxides enzyme, also activity enzymes glutathione reductase, glutathione peroxides and total antioxidant increased with added Vit E but superoxide dismutase didn’t influence. Level of testosterone and estrogen hormones significantly increased by supplement Zn. Level of cholesterol by added Zn and also level triglyceride and cholesterol by supplement Vit E decreased. Results this experiment indicted supplementation zinc and Vit E effective on antioxidant enzyme, sexual hormone and some biochemical parameters