Effect of Bacillus coagulans and its culture extracts on cell cytotoxicity of Clostridium perfringens type A on HT-29 cell line

The present study was carried out to investigate the effect of Bacillus coagulans Gbi-30 and its culture extracts on the cytotoxicity induced by vegetative form of Clostridium perfringens type A on the HT-29 cancer cell line. For this purpose, extracts with concentrations of 2, 4, 6 and 8% of the supernatant derived from Bacillus coagulans culture in TSB broth medium were added to the vegetative form of Clostridium perfringens type A (NCTC 8239), to the HT-29 digestive cells. For cytotoxicity assessment, MTT and trypan blue staining assays were employed. The results showed that cultivation of Bacillus coagulans and its 2, 4 and 6% extracts were significantly reduced the cytotoxicity of Clostridium perfringens type A using both MTT and staining trypan blue staining. In addition, the lowest cytotoxicity was observed on the presence of the 4% Bacillus coagulans extract on the HT-29 cell line.