Evaluation of antibacterial effect of the combination of Bentonite nanoclay and essential oils of Cinnamomum zeylanicum on Escherichia coli and Salmonella in Vitro

Calf diarrhea diseases result from complex interactions of the infectious and non-infectious agents and is one of the most common diseases reported calves up to 3 months old. Among the bacterial causes of diarrhea Escherichia coli and Salmonella species are the most common. Concurrent with the development of new chemical drugs and antibiotics, their harmful effects are gradually emerged. Nowadays the research works results show that herbal medicines have not any harmful effects. The goal of this study is synergic effects of Bentonite Nano clay and essential oils of Cinnamomum zeylanicum on Escherichia coli and Salmonella in Vitro. For determination the minimum inhibition concentrations (MIC) of essential oils of Cinnamomum zeylanicum, and Bentonite nanoclay was investigated individual and combination with each other on two causal agents of bacterial diarrhea in BHI broth media by macro-dilution method. The result showed that the MIC of essential oils of Cinnamomum zeylanicum was 0.01% and 0.02% for Salmonella and Escherichia coli, respectively. Meanwhile the MIC of essential oils of Cinnamomum zeylanicum combinated with Bentonite nanoclay was 0.01% and 12.5 mg/mL for Salmonella and 0.02% and 25 mg/mL for Escherichia coli. In conclusion, essential oils of Cinnamomum zeylanicum individual and also combination with montmorillonite nanoclay have a good accurate to control on two major agents of bacterial diarrhea.