A Review on Parameters Influencing Dark Fermentative Biohydrogen Production

Dark fermentative biohydrogen production is a complex multiproduct process and is affected by many parameters. In the present review, effect of a wide range of parameters such as inoculum, temperature, pH, hydraulic retention time, bioreactor configuration, hydrogen partial pressure, substrate type and concentration, nutrient type and concentration in dark fermentative biohydrogen production is addressed. Most of the studies on dark fermentative biohydrogen production have been conducted in batch mode using glucose and sucrose as substrate, thus future studies in continuous mode using organic wastes and inexpensive substrates are desirable.