Molecular typing SCCmec cassette chromosome in methicillin resistant Staphylococcus aureus strains

Staphylococcus aureus is one of the most important pathogens acquired of hospital and community. The aim of this study is molecular typing SCCmec cassette chromosome genes in S. aureus strains resistant to methicillin. In this study 106 clinical isolates of Staphylococcus aureus were collected of hospitalized patients in the milad hospital. Antibiotic susceptility test was performed in 106 isolates of Staphylococcus aureus by oxacillin and cefoxitin by disk diffusion method according to CLSI protocol. Fifthy resistant strains were isolated. DNA Extenation of resistant strains were performed by boiling method, SCCmec genotype was determined by PCR and multiplex PCR.A total of 50(47.15%) isolates of S.aureus were MRSA. PCR and typing showed that type I 11 (22%), type II 20 cases (40%), type III (28%), type IVa 7 (14%), type IVb 5 (10%), type IVc 12 ( 24%), type V 9 (18%) were observed and SCCmec type IVd was not observed in isolates. Different Sccmec type is related to the origine of strains isolation and differ in susceptibility to Antibiotics. In this study, type IVa was least Sccmectype and type III and type II were most common genotype in strains, which induce multi drug resistance in strains.