Phylogenetic Characterization oF Endophytic Bacterium Pseudomonas Protegens with Antagonistic Ability Isolated From privet

Endophytic bacteria are able to control plant pathogens by producing antagonistic compounds. This research was conducted to determine the antagonistic ability of bacterial endophytes in privet (Ligustrum vulgare L.). In this study, endophytic bacteria were isolated from stem and leaf of privet (Ligustrum vulgare L.). After genomic DNA extraction, 16S rDNA gene was amplified using polymerase chain reaction (PCR) technique for precise bacterial identification. Then, the PCR product was sequenced by BLAST. Strains were examined for siderophore, hydrogen cyanide, and protease tests. Findings: All the isolated bacteria were able to produce protease. Only one strain (BN3) was able to produce hydrogen cyanide. The siderophore test was positive for one strain (BN2). Based on the 16S rDNA sequence studies, the strain bacteria belonged to Pseudomonas protegens and indicated 100% similarity to type strain. Discussion & Conclusions: The endophytic bacteria isolated in this study can be used to promote plant growth. Knowledge about endophytic bacteria-plant interaction can provide an effective strategy to develop sustainable agriculture in order to ensure yield improvement without affecting environment.