Effects of Different Media Factors on In Vitro Proliferation of St. John’s Wort (Hypericum perforatum)

St. John’s wort is a traditional medicinal plant that has been used from ancient time for the treatment of neurological and psychiatric diseases. A successful in vitro propagation system for the St. John’s wort was optimized. Different explant type (single node and stem cutting with two nodes) were used in Murashige and Skoog (MS) medium in different concentrations (MS full, 3/4 MS and 2/3 MS) supplemented with BA (0.05, 1.0, 2.0 mgl-1) and sucrose (30 and 45 gl-1). The optimum proliferation rate of St. John’s wort was obtained in 2/3 MS medium containing 30 gl-1 of sucrose and 0.1mgl-1 BA with single node explant. Also, evaluation of results revealed that the number of proliferated shoots will increased in MS media supplemented with BA along with reduced concentrations of sucrose. The in vitro generated shoots were cultured on half-strength MS medium containing 0, 0.2, 0.4, 0.6, 0.8 and 1 mgl-1 butyric acid (IBA). The results showed that all proliferated shoot were rooted in plant growth regulators free medium. But, using and increasing the concentration IBA in rooting media can increases the number of roots and average of root length per each explant.