Studying expression of leptin gene in different tissues of Kermani Sheep using Real Time PCR

Leptin is produced by white adipose tissue and plays an important role in regulation of feed intake, energy balance, fertility and immune functions. The aim of this research was to study leptin gene expression in adipose tissue, liver, kidney, lung and heart of Kermani sheep. Tissue samples obtained from 6 animals and RNA was extracted. Extracted RNA were immediately stored at -80°C. Quality and quantity of RNA were evaluated and cDNA was synthesized and Real Time PCR was performed. PCR Products were electrophoresed on 1.5% agarose gel and were evaluated different levels of expression in studied different tissues. Results showed that the leptin gene was expressed in all the tested tissues and the highest level of expression was observed in adipose tissue and liver and the lowest level was detected in heart. These results may show that leptin plays a particular role in fat metabolism. Further studies are needed to clarify role of leptin in the physiology of fat metabolism and other materials. This would help us to better understand the mechanisms for the known effect of nutritional factors and body fatness on various functions.