Study of yield, vegetative traits and seed germination of pinto bean produced from biopriming with Trichoderma

Increasing germination percentage and growth indices is important by using methods such as biopriming. The present study aims to investigate effect of biopriming with a number of selected species of native Trichoderma fungi of Iran on yield, germination, vegetative and morphological components of Talash vriety bean seedlings. Also, the effect of induced-mutation by gamma irradiation on the Trichoderma genome and probebly increase in the efficiency of biopriming via using mutated Trichoderma isolates was studied. Experiments with three treatments: control (non-inoculation), Inoculated with wild type Trichoderma based bio-formulation and inoculation with mutants Trichoderma, at the Nuclear Agriculture Research Institute in the form of a completely randomized design with four replications were done. Evaluation of indices (Germination percentage; seedlings, stems and roots length ; their fresh and dry weight and vigor index) based on the ISTA standard method and yield in greenhouse was implemented. Results showed, bean seeds biopriming method with bio-formulations of Trichoderma species (wild type and mutant), plant's yield increased significantly compared to control. Germination percentage of seeds was significantly higher than control in fungal treatments. The important trait of dry weight has also increased compared to control and also had significant difference with control. The length of seedlings of bioprimed with Trichoderma was shorter than the control. Most of the indicators were significantly higher in the one percent level than control. Overally, the results of this study shows, in bean plants, the use of bio-priming seeds with Trichoderma, improves plant growth parameters especially in establishment and its early growth.