A Review on the Effect of Chemical and Physical Mutagens in Increasing Dark Fermentative Hydrogen Production by Enterobacter Strain

Dark fermentative hydrogen production is an elaborated multiproduct process that takes place in the absence of light and oxygen. In present paper effect of chemical and physical mutagens on increasing dark fermentative hydrogen production using Enterobacter strain is reviewed. The hydrogen production rate of mutant compared to the wild strain was increased and other metabolite production was decreased by using both chemical and physical mutagens. The various techniques such as testing sugar utilization efficiency, suicide substrate, suicide protons and Voges–Proskauer was used for screening mutant cells. However, the hydrogen production rate as index for selection the best mutant has been considered. Hydrogen production by using chemical mutagens was higher than physical mutagens.