Control of Inhibitory factors in the Bio-refinery of bagasse pith

In this study, in order to control the inhibitory compounds in the bio-refinery, bagasse pith was pretreated under acidic hydrolysis with dilute sulfuric acid 8%, 90 minutes at 120 ° C. Then, by using de-depleting methods of overliming, activated carbon and combining of both, removal of deterrent compounds from the above pretreatment hydrolyzates was performed. In the overliming, calcium hydroxide and sulfuric acid, in activated carbon method, activated carbon was used at three levels (0.5, 1.5 and 2.5%) and in the combined method of the combination of materials in the two previous methods became after acid treatments and each of the methods of insemination, the amount of recovered sugars and the values of the inhibitory compounds of furfural and hydroxymethylfurfural in the hydrolyzate were measured as two important indicators. The results showed that application of the above methods on hydrolyzate increased the yield of reduced sugars, so that the highest sugar yield (˃32%) was obtained after the independent method of overliming. In addition, active and combined carbon autonomous methods (overlimming and activated carbon) have an optimum activated carbon consumption of 2.5 and 1.5%, respectively. The qualitative study of the presence of inhibitory agents in hydrolyzates showed that furfural and hydroxymethylfurfural have maximum absorption at wavelengths of 276 and 282 nm. Also, an overliming with 2.5% activated carbon as the best method could eliminate up to 100% of inhibitory compounds. On the other hand, the autonomous method of overliming, with the removal of ˃90% of the inhibitory compounds, also has the highest sugar yield. Therefore, taking into account simultaneously the rate of sugar yield and the elimination of deterrent compounds, two independent alternatives of Overliming and Overliming with 2.5% active carbon could be proposed for the production of bioassays, such as bioethanol, xylitol, ... from the pith of bagasse.