Correlation of Plasma Lipids' Profile with Testicular Histology Parameters in Broiler Breeder Roosters

The aim of this study was to determine correlation of plasma lipids profile with testicular histology parameters in Ross-308 broiler breeder roosters. This study was performed on 12 male ross 308 broiler breeder. Blood samples were collected from a wing vein to measure plasma lipid profiles (cholesterol, triglyceride, LDL and HDL lipoproteins, cholesterol, and glucose). The concentrations of cholesterol, triglyceride, LDL, HDL were measured by spectrophotometric method using commercial kits of pars azmoon co. though, glucose concentration was measured by electrochemical method using glucose monitoring system. For testicular histology study (endocrine parameters of the testis); the 5 μm paraffin cross sections of testis tissue was used. The data were analyzed by the Pearson correlation test. Our results showed that the plasma HDL values were positively correlated with the number of leydig cells (r=0.73; p<0.01), the number of spermatogonia cells (r=0.92; p<0.001), seminiferous epithelium thickness (r=0.86; p<0.001) and diameter of seminiferous tubules (r=0.62; p<0.001). Also, plasma cholesterol and glucose values were negatively correlated with the number of the leydig cells (r=-0.78; p<0.01؛ r=-0.69; p<0.001), spermatogonia cells number (r=-0.68; p<0.001؛ r=-0.72; p<0.01), seminiferous epithelium thickness (r=-0.80; p<0.01؛ r=-0.82; p<0.001), and diameter of seminiferous tubules (r=-0.80; p<0.01؛ r=-0.82; p<0.001). Plasma LDL value also had a negative correlation with the number of spermatogonia cells (r= -0.39; p<0.001), and also, diameter of seminiferous tubules (r= -0.69; p<0.01). Plasma triglyceride values was negatively correlated with the number of spermatogonia cells (r= -0.79; p<0.001), and seminiferous epithelium thickness (r= -0.76; p<0.01). In conclusion, HDL lipoprotein had a positive correlation with testicular histology indexes, on the other hand, triglyceride, cholesterol, lipoprotein LDL, and glucose showed a negative correlation with that.