Evaluation of the Effects of Curcumin Nanoparticles on the Expression of Genes Involved in Biofilm Formation in UPEC

  Uropathogenic Escherichia coli (UPEC) is one of the most important causative agents of urinary tract infection (UTI). UPEC isolates persist in the body through biofilm formation. The successful adhesion is the most important step of biofilm formation. Type 1 and P are bacterial surface appendices, which play a pivotal role in of UPEC. The aim of this study was to assess the effect of on the initial adhesion gene expression in UPEC isolates. The presence of and genes among 60 UPEC isolates was investigated by PCR; 5 potent producer UPEC strains from patients with UTI were exposed to the sub-minimum inhibitory concentration of Expression of the and genes was evaluated by real-time PCR. Of the 60 UPEC isolates, biofilm formation was seen in 27 (45%) of isolates, 5 of which produced strong The result of PCR assay showed that was seen in 57 (95%) of the 60 UPEC isolates and was seen in 58 (96.6%) of isolates, respectively. and expression 7 and 8 fold in all 5 isolates, respectively. Sub-MIC concentrations of remarkably decreased the expression the and genes in strong forming UPEC strains, but cannot prevent biofilm formation.