Evaluation gibberellin and auxin production ability by endophytic bacteria Pseudomonas fluorescens in soybean L17 cultivar

Endophytic bacteria live inside tissues of their plant host without causing visible symptoms, and in more cases are reported as plant growth promoting bacteria. This research was conducted to determine plant growth promoting affects of endophytic bacteria associated with Soybean (cultivar L17). In order to isolate endophytic bacteria from various parts of soybean (cultivar L17), samples were collected from the research farm of Agriculture faculty in this research endophytic bacteria were isolated from leaf, stem, seed and root of. After genomic DNA extraction, 16S rDNA gene was amplified using PCR. Then, the PCR product was sequenced by BLAST. Strains were surveyed for IAA and GA production ability was carried out using a randomized complete design in three replications. Analysis of variance and mean comparison was performed using Duncan Test with SAS software. The isolated bacteria were able to produce IAA in various amount 20/03 - 45/33 without tryptophan and in the presence of it, 26/76 -51/17 microgram/milt. GA producing abilities were also 12/97-17/15 microgram/milt for these isolates. Based on the 16S rDNA sequence studies, this bacterium belonged to Pseudomonas fluorescens and indicated 99% similarity to type strain. This study is the first report of isolation of Pseudomonas fluorescens from Soybean (L17 cultivar). The endophytic bacteria isolated in this study can be used to promote plant growth.