The Study of Antimicrobial and Anti-Enzymatic Activity (Potato Poly Phenol Oxidase) of Cinnamomum Verum Hydrosol: A Laboratory Study

Cinnamon contains phenolic and non-phenolic bioactive compounds. The aim of this research was investigating the antimicrobial and anti-enzymatic properties of cinnamon (Cinnamomum verum) hydrosol. In this laboratory study, chemical composition of cinnamon hydrosol was analyzed by Gas chromatography–mass spectrometry method.  Antibacterial effects of cinnamon hydrosol at different concentrations (10, 20, 30, 40 and 50 percent) against Staphylococcus aureus and Bacillus cereus and antifungal effects against Aspergillus niger and Rhizospus oryzae were determined. Anti-enzymatic activity of cinnamon hydrosol against the polyphenol oxidase enzyme of potatoe was also assessed. The experiment was designed in a completely randomized design with three replications. The data were analyzed using one-way ANOVA and Duncan's mean comparison test. The results showed that Bacillus cereus has high susceptibility against cinnamon hydrosol (the mean and standard deviation of inhibition zone diameter equal to 37.83±0.21mm). Investigation of anti-fungal activity revealed that  Aspergillus niger showed more stability against cinnamon hydrosol than  Rhizospus oryzae (the mean and standard deviation of the growth  inhibition percentage at hydrosol concentration of 50% w/v, were 9±2.2 and 68.66±2.5, respectively). Cinnamon hydrosol caused a significant reduction in the polyphenol oxidase activity (p˂0.001). According to this study, cinnamon hydrosol exhibited anti-bacterial and anti-fungal properties as well as, anti- enzymatic activity against polyphenol oxidase of potato, so that cinnamon hydrosol can be used as antibacterial (against Bacillus cereus), antifungal (against Rhizopus oryzae) and anti-enzymatic (poly phenol oxidase) component. Key words: Hydrosol, Cinnamon, Antimicrobial effect, polyphenol oxidase   Funding: This study did not have any funds. Conflict of interest: None declared. Ethical approval: The Ethics Committee of Islamic Azad University, Neyshabur Branch approved the study (Ethics number:1397.012).   How to cite this article: Didar Z. The Study of Antimicrobial and Anti-Enzymatic Activity (Potato Poly Phenol Oxidase) of Cinnamomum Verum Hydrosol: A Laboratory Study. J Rafsanjan Univ Med Sci 2019; 18 (1): 3-16. [Farsi]