Identification of Salmonella and E.coli with invA and lamB Genes by Multiplex PCR in Chicken Samples

Salmonella and Escherichia coli are the most common food pathogens bacterial especially in chicken products. Rapidly, specifically and simultaneously detection of the pathogens by identification of special genes, is important. This study aim was identification of both E.coli and Salmonella in chicken products by using multiplex PCR with lamB and invA genes amplification. 100 chicken samples were obtained . Samples were washed by sterile saline water and were cultured in biochemical medium. Salmonella and Escherichia coli strains were purified and DNA extraction was performed. Then amplification of both invA and lamB genes were used by multiplex PCR. From 100 chicken samples, 9 samples and 26 samples were isolated by biochemichal tests, Salmonella and E.coli, respectively. Multiplex PCR results showed ferequency of invA gene was 9% and for lamB gene was 26% , As also, frequency of both genes in samples were 1%. In this study, multiplex PCR results was based on Salmonella and E. coli special virulence genes was comfirmed biochemical tests results. Therefore comparation of results showed Multiplex PCR for rapid and simultaneous detection of food pathogens has high sensitivity and can be a reliable method for controlling of food contamination.