Feasibility of Film Based Sodium Caseinate-Nanoclay and Echinophora Platyloba Essential Oil useing in packaging of probiotic yogurt

In this study the caseinat sodium film containing nanoclay and Echinophora Platyloba essential oil was used in order to possibility of using biodegradable packaging as well as  its effect on the viability of probiotic bacteria to cover the contents of yogurt. The samples were stored at 4 and 15 ° C for 21 days. The effect of packing material on the survival of Lactobacillus acidophilus was not significant, while on the growth of Bifidobacterium was significant. The highest number of bacteria was observed in the coating containing 1.5 grams of nanoclay and the lowest number of containers containing 0.75 grams of nanoclay. By increasing storage temperature and time, simultaneously, the process of decreasing the number of live bacteria was accelerated .the reduction of Lactobacillus acidophilus bacteria at 15 ° C after three weeks of storage / 0 was a logarithmic cycle, while in the same situation, the number of Bifidobacterium analisis bacteria decreased by 1.3 times, indicating a higher resistance of Lactobacillus acidophilus to acidic conditions of yogurt during the storage time. At 4 ° C, there was no difference in mean pH and acidity between different packaging samples, but there was a significant difference between all packaging samples at 15 ° C. The highest decrease in pH and maximum acidity was due to the packing of 1.5 g of nanoclay in the end of the storage time