Effects of ProbioEnzyme on some growth performances, hematological indices and carcass quality of grass carp fry

This study evaluated the effects of different levels of probioenzyme™ (including four probiotic strains; Bacillus Lichenformis, B. subtilis, Entrococcus faecium, and Lactobacillus acidophilus with five enzymes α-glucanase, β-glucanase, α-xylanase, α-amilase, protease, and cellulase) on some growth performance, hematological indices and carcass proximate composition of grass carp fry. 120 fish (average weight of 7±0.5 g) were randomly divided into 12 fiberglass tanks. The fish were fed with three concentrations of probioenzyme including: 0 (control group), 1, 2 and 3 g/kg of feed for 45 days at 23.59±1.2 ºC. At the end of the trial, the hematological parameters were measured. The results showed that the use of probioenzyme increased the growth parameters and feed efficiency between treared groups compared to control one (p<0.05). The highest level of weight gain (10.02±0.22 g), specific growth rate (0.96±0.8 %/day), protein efficiency ratio (24.50±0.03) and condition factor (1.28±0.13 %) and the lowest level of feed conversation ratio (1.20±0.11) was observed in of 3 g of probioenzyme. Also, the level of WBC, monocyte and lymphocytes was not significantly difference between experimental treatments (p>0.05). However, the level of RBC, Ht and Hb were significantly increased in 3g probioenzyme compared with others (p<0.05). Also, the level of lipid and ash contents of the fish fleh showed not any significant differences between experimental treatment (p>0.05). While, the highest content of protein (14.33±29%) was observed in 3 g probioenzyme and the highest level of moisture (83.82±0.38%) was observed in 2 g probioenzyme per kg of feed (p<0.05). In conclusion, oral admistration of 3 g probioenzyme per kg of feed had a positive effect on growth performance, hematological indices and carcass quality of grass carp fry.