Isolation and Identification of Lactic Acid Bacteria from Gastrointestinal System of Honeybee in Markazi Province

Honeybee is a useful insect who plays a critical role due to pollination. Gastrointestinal system of honeybee has coexistent microorganisms. The recent researches showed that the useful microorganisms in honeybee are recognized to participate in the interactions of honeybee body. Using probiotic supplements in apiculture industry will increase the speed of growth, improve coefficient of food transformation, improve the resistance to disease and increase the honey production. The purpose of this research is to separate the useful bacteria of gastrointestinal system of honeybee in several districts of Markazi Province and study the probiotic indices of samples. Accordingly, the samples of healthy worker bees were separated from apiaries and carried to the laboratory for separation of gastrointestinal system. They were heated and enriched in MRS broth under anaerobic condition. Colonies of negative catalysis and hemolysis were analyzed by phylogenic diagnosis test. One lactobacillus was tested for the phylogenic identification which was lactobacillus alimentarius. The results of this research indicated that presence of lactic acid bacteria in gastrointestinal system of honeybee.