The Effect of 12 Weeks Aerobic Training with and without Consumption Multivitamin Minerals Supplementation in Polluted Air on Some Immunological Factors in Healthy Men

The purpose of this study was to examine the effects of 12 weeks of aerobic training with and without consumption multivitamin minerals supplementation in polluted air on some immunological factors in healthy men. 46 healthy men (age: 25 ± 2.2; BMI:23.83 ± 0.98 kg/m2</sup>), were randomly assigned into 4 groups: aerobic training with multivitamin minerals supplement (AT+MS, n=11); aerobic training only (AT, n=11); multivitamin minerals supplement only (MS, n=12) and control (C, n=12) in polluted air with an average unhealthy index. The exercise program consisted 90 min aerobic training, 3 times weekly on non-consecutive days at 60-85% of heart rate reserve (HRR) for 12 weeks. Participants in the AT+MS group were additionally asked to consume 500 mg multivitamin-mineral supplement daily for 12 weeks. White blood cells, neutrophils, lymphocytes, monocytes, eosinophils and basophils, cortisol levels, and salivary immunoglobulin A were measured by cell Counter, ELISA and immunodefusion techniques in baseline and at the end of 12 weeks of intervention. The data were analyzed using repeated-measures analysis of variance. After intervention, there was significant increase in the number of all immune cells (P=0.01) cortisol levels (P=0.047) in the AT group and a significant decrease in the number of lymphocytes (P=0.001), monocytes (P=0.01) and basophile (P=0.041) in the MS group. Also, the s-IgA levels increased significantly (P=0.047) in the AT+SM group, but remained unchanged in the other groups. The results of the study showed that aerobic training combined with multivitamin minerals supplementation causes improvement in some of the immunological factors in the polluted air. However, to achieve more conclusive results, further investigation is needed.