Lethal effects of insecticides clothianidin, tebufenozide and flupyradifurone on egg, 2nd and 3rd instar larvae and pupae of green lacewing, Chrysoperla carnea Stephens

The green lacewing, Chrysoperla carnea</em> Stephens (Neo., Chrysopidae) is one of most important predators of aphids, mealybugs, thrips and whiteflies. Moreover, fed on egg, young larvae and pupa of lepidopterons and mites but when chemical treatment on various insect pests, the green lacewing is also influenced by different agricultural pesticides. In current study, lethal effects of three insecticides; clothianidin, tebufenozide and flupyradifurone on egg, 2nd</sup> and 3rd</sup> instar larvae and pupae of green lacewing, C. carnea</em> were evaluated. Bioassay of egg and pupa of green lacewing with dipping method but 2nd</sup> and 3rd</sup> instar larvae with contact method were carried out. All experiments were conducted at controllable conditions of 25±1 °C, 65±5 % and a photoperiod 16: 8 h (L: D). The results revealed that LC50</sub> of clothianidin, tebufenozide and flupyradifurone on egg were 23.41, 1048.95 and 774.95 mg/lit; on 2nd</sup> instar larvae were 12.47, 476.64 and 761.78 mg/lit; on 3rd</sup> instar larvae were 23.47, 490.35 and 855.09 mg/lit and on pupal stage were 37.41, 1401.95 and 796.82 mg/lit. Therefore, in all stages the highest sensitivity was due to clothianidin insecticide while the lowest sensitivity in egg and pupa stages associated to tebufenozide but in 2nd</sup> and 3rd</sup> instar larvae were related to flupyradifurone. Thus, based on results of this study, conclude that from insecticides tebufenozide and flupyradifurone can be utilized in program of IPM under application green lacewing.