Micropropagation of Narcissus tazetta L. treated with different hormones and transfer of plantlets to the soil

Narcissus tazetta L. of the Liliaceae family, despite the high demand market has very slow vegetative propagation. For the rapid propagation, in this study micropropagation of Narcissus under different hormones and soil transfer of plantlets was investigated. Micropropagation of this plant was done in three procedures: induction, proliferation and bulb production from explants. It was found that the best medium for shoot induction is modifiedMS+ 4 mg l-1 BAP +0.12 mg l-1 NAA and shoot clumps which were cultured on the MS+2 mg l-1 BAP +0.12 mg l-1 NAA medium, showed the most proliferation. Transferring these shoot clumps to MS+ 9% sucrose + 0.1 mg l-1 NAA medium resulted in maximum bulbs production. After 4 months, the bulbs were 12–20 mm in diameter. In this medium, roots were thicker and shorter than the ones grown without NAA. The bulbous plants treated with salicylic acid and transferred to soil and then treated with Carbendazim fungicide. Carbendazim increased survival percent (100%) but salicylic acid did not have any effect on success of transferring to soil. The high number of bulbs and high percentage of survival of regenerated plants in soil indicated that micropropagation is an effective alternative method compare to traditional method.