Effect of sperm activating solutions on the performance of artificial reproduction in Salm o trutta caspius at the mid of propagation season

The appropriate quality of sperm whose important feature is movement , can cause increased fertilization. As the quality of sperms and ova were reduced at the end of the propagation period, and this problem may cause quality decrease of production, this research has been conducted in order to increase the efficiency of reproduction fertilization, by using activating solution at the mid of propagation season at the salmonid reproduction and conservation center of ShahidBahonar–Kelardasht in late 2012.In this regard, firstly 14 activating solutions with different component have been selected and were used in preliminary studies and finally, 7 activating solutions on the basis of compound and duration of sperm movement have been chosen for final surveys. In order to survey of the 7 selected solutions as well as distilled water, hatchery water (As control group) and physiology serum , on the duration of sperm movement, fertilization rate, eyed egg and hatching, 5 females and 5males of rainbow trout were randomly selected.Results showed that, among surveyed items, the activating solutions had significant difference comparing with each other and also the control group.The highest duration of sperm movement was produced by the number 1 (NaCl = 6gr/L,CaCl2.2H2O = 0.2gr/L , CO(NH2)2= 4.50gr/L, pH=7.6) and 6 (CaCl2.2H2O=./735 gr/L,NaCl=7/305 gr/L ,pH=7/7)Activating solutions which have significant difference with the other groups and also the control group. But fertilization, eyeing and hatching rate have not significant different with control group. Final conclusion confirmed that, the sperm activating solutions have positive and significant effects on extending the time length of sperms movement of Salmo caspius but there was no significant differencein surveyed items consist of fertilization, eyeing and hatching rates comparing with control group.