Investigation of Biodegradation of Cyanide by Secretory Nitrilase Enzyme in Penicillium

The cyanide is a toxic and very lethal compound that has devastating effects on the environment and human health. Physical and chemical methods are very costly to remove contamination in high large areas. This fact leads to understanding of the potential of microorganisms, including fungi, in the effective and economical purification of soil and contaminated water. In this study, a random sample was taken from a gold mining wastewater. Samples were cultured in a PDA medium. Then, to evaluate the ability of fungus to measure the fungal power in cyanide decomposition, Picric acid method was used. Take In this study, the specific activity of the cyanide degrading enzyme called nitrilase in concentrations of 0, 2, 5 and 10 mM cyanide was investigated in Penicillium fungus culture media. To determine the nitrilase activity of the spectrophotometer and to absorb benzoic acid at 238 nm. Benzonitrile produces benzoic acid and ammonia in the presence of nitrilase enzyme. With the help of the standard curve obtained from the absorption of benzoic acid, the activity of the nitrilase enzyme was obtained. To analyze the significance level of data, ANOVA and T. test tests were used. The results indicate an increase in the specific activity of this enzyme in conjunction with an increase in the concentration of cyanide in the culture medium. As a result of a concentration of 0 to 10 mM cyanide, the specific activity of the nitrilase enzyme increased by 26%.