In vitro evaluation of the anti-bactrial and anti-oxidant properties of Coelomocytes and its extracts (organic phase and aquatic phase) isolated from Echinometra matheai

In this study, anti-bacterial and antioxidant activities of coelomocytes and aqueous and organic extracts of Sea urchin were investigated. The live samples of sea urchin were collected from Persian Gulf and their coelomocytes and extracts were isolated. Antibacterial effects of coelomocytes and its extracts were assessed on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa. The results of this study showed that coelomycetes and extracts had strong antibacterial effects. The most powerful antibacterial effect was related to the aqueous phase. Also, antioxidant activitiy (DPPH and ABTS free radical as well as reducing power assay) showed that aquatic phase had highest DPPH scavenging activity (at a concentration of 1/25 mg/ml) and organic extract had the highest effect on ABTS scavenging (at a concentration of 5 mg/ml). Reducing power assay indicated that extracts showed the lowest activity and there was no significant effect in any concentrations (α ≥ 0). MIC and MBC of the bioactive compounds in this study were equal to each other. MIC and MBC for the coelomocytes, aqueous and organic extracts on experimental bacteria (Staphlococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa) was obtained in 5 > , 2.5 , 2.5 , 5 and 2.5 , 0.019 , 0.019 , 0.019 and 5 > , 0.625, 0.625 & 2.5 ug/ml, respectively. Generally, according to the antibacterial and antioxidant results of the study, the extracts from sea urchin can be used as packaging, antimicrobial and antioxidant agent.