Molecular detection of inclusion body hepatitis (IBH) agent in broiler chickens in Isfahan province

Avian Adenoviruses are widely distributed in commercial flocks in the world and show wide variety of virulence and clinical signs. Some of isolates of fowl adenoviruses can cause Inclusion Body Hepatitis (IBH). The importance of IBH agents are economic losses and its association with other viral diseases. Immunosuppression after Infectious Bursal Disease (IBD) can facilitate disease of IBHV.Also simultaneous infection with chicken infectious anemia virus(CIAV) and IBH increase the heart injuries and mortality. Mortality rate in IBH can reach to 10% but sometimes it can be over 30%. In this study identification of IBHV by PCR method and presence of Hexon gene in variable area of Loop1 was studied in Isfahan province for the first time. 69 liver samples from suspected 35 flocks and cities gathered and sent to laboratory. Five samples from Kashan, Ardestan and Golpayegan were positive in PCR. So presence of IBHV confirmed in this province.