Characterization of Mutations in the Rpob and Katg Gene of Mycobacterium Tuberculosis Isolates From Pasteur Institute of Tehran

The Rifampicin resistance and susceptibility of Mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpoB gene encoding the b-subunit of RNA polymerase.

Isoniazid resistance of M. tuberculosis is related to mutations in inha , oxyR and ahpC genes which 30 to 90 percent of Isoniazid resistance is occurred in 3015 codons of katg gene. The rpoB and katG sequences of 30 isolates were analyzed to identify the mutations and compare the mutations with their related susceptibilities.

In this research, we investigated the location and type of rpoB and katG mutations in Mycobacterium tuberculosis which had been achieved from Pasteur Institute of Tehran. PCR Amplification and DNA sequencing methods were performed. In this assay, from 507 to 537 codons and 315 codons of rpoB and katG genes were sequenced and also mutations were analyzed, respectively.