Trans-Differentiation of Human Dental Pulp Stem Cells Into Cholinergic-‎Like Neurons Via Nerve Growth Factor

Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Introduction

Cell therapy has been widely considered as a therapeutic approach for neurodegenerative diseases and nervous system damage. Cholinergic neurons as one of the most important neurons that play a significant role in controlling emotions, mobility, and autonomic systems. In this study, human dental pulp stem cells (hDPSCs) were differentiated into the cholinergic neurons by β-mercaptoethanol in the preinduction phase and also by the nerve growth factor (NGF) in the induction phase. 

Methods

The hDPSCs were evaluated for CD73, CD31, CD34, and Oct-4. Concentration-time relationships for NGF were assessed by evaluating the viability rate of cells and the immune response to nestin, neurofilament 160, microtubule-associated protein-2, and choline acetyltransferase.

Results

The hDPSCs had a negative response to CD34 and CD31. The optimal dose for the NGF was 50 ng/mL seven days after the induction when the highest percentage of expressing markers for the cholinergic neurons (ChAT) was detected.

Conclusion

The results of this study provided a method for producing cholinergic neurons by hDPSCs, which can be used in cytotherapy for degenerative diseases of the nervous system and also spinal cord injury.

Language:
English
Published:
Basic and Clinical Neuroscience, Volume:10 Issue: 6, Nov-Dec 2019
Pages:
609 to 618
https://www.magiran.com/p2100098