Isolation and identification of phytase producing fungi for extraction and use of enzymes in animal feed and poultry

In the present study, native fungi producing phytase from soil and other environmental samples (Includes: soil around the root of leguminous plants, Cows and goats excrement, Chicken floor, Vermicompost, Mineral phosphorus mine, Sewage water, Plants and fruits infected with fungi and Samples prepared from research centers of the country)were isolated and incubated for 5 days at 28 ° C on SDA(Saburad Dextrose Agar). Then a phytase screening medium (PSM) was used to identify and isolate fungi capable of producing phytase enzyme. Morphological characteristics of fungal strains were studied using optical microscopy.Samples which produced clear halo were chosen for enzymatic activity assay based on the release of a phosphorus nanomol in one minute. Based on the amount of enzyme and amount of biomass production, were evaluated by the respective isolates.The optimum pH and temperature for maximum production of enzyme through the selected isolates were 5.5 and 30 °C, respectively. Finally, the isolates were identified as Fusariumoxysporum and Aspergillus Niger using Genetic analyzer system. The maximum amount of enzyme production was recorded as 130.11 and 125.27 enzymatic units for Fusarium oxysporum and Aspergillus Niger, respectively. The results showed that from five to seven days during enzyme culture are the most suitable time for enzyme extraction and purification.The results of morphological studies were completely consistent with the molecular identification results