(Short Paper)Study on the effect of different concentrations of colchicine on the secondary metabolites production in Linum usitatissimumcalli (Flax)

 In this factorial study in a completely randomized design, the effect of different concentrations of colchicine (0, 15, 30, 45 and 60 mg/L) and different time intervals (12, 24 and 48 hours) on the amount of phenol and lignan production was investigated in the callus tissue of Linum usitatissimum L. For callus induction, cotyledon explants were cultured on solid MS medium supplemented with 2 mg/l 6-Benzylaminopurine (BAP) and 1 mg/l 1-Naphthaleneacetic acid (NAA).The total phenolic and lignan contents were measured by spectrophotometric method based on the standard curves of gallic acid and sesamin at 765 and 288 nm, respectively. The highest amount of fresh and dry weight of callus was related to 45 mg/l colchicine treatment. Maximum and minimum levels of lignin in experimental periods were obtained from 15 mg/l (in average 28.4 mg SE/ g DW) and 30 mg/l (in average 19.75 mg SE/ g DW) colchicine, respectively. According to the results, the highest (164 mg GA/g) and lowest (145.6 mg GA/g) levels of total phenol content were observed in 60 and 30 mg/l colchicine treatments, respectively. The results indicated that the production of valuable secondary metabolites such as phenol and lignan in flax callus were increased under some colchicine treatments.