In this experiment, six treatments were performed in a completely randomized block design and in three replications as follows: T1 = control (no fertilization); T2 = fertilization based on farmers conventional fertilizer use (NP); T3 = balanced fertilization (fertilizer recommendation on the basis of soil test); T4= T1+ bio- fertilizer; T5 = T2+ bio-fertilizer and T6= T3+ bio-fertilizer. Samples were taken at two- time intervals. The samples were weighed in dry content. Meanwhile, essential oil extractions were measured by Clevenger Instrument and total antioxidant extracted by DPPH method. Subsequently, data were analyzed by SAS software. The differences were shown significant at 5 percent statistical level. Average yield of dry leave weight and essential oil in two harvest in control treatment were 664 and 7.15 kgha-1, respectively, these values in the best treatment were 1261 and 19.97 kgha-1, respectively. The treatment of balanced fertilization + bio-fertilizer compared with other treatments has the highest amount of antioxidant properties in both harvest. Also, the highest concentration of potassium, iron, zinc and manganese in edible organs (Leaves) in both the yields was found in balanced fertilization + bio-fertilizer. Also results analysis of essential oil showed that percent of citral (neral+geranial) in essential oil in control treatment were 40.52, these value in the T6 treatment wrer52.27.