Impact of Nano-Fe fertilizer rates on germination traits and protein pattern of three Mellisa offisinalis species

Genera of Lemon balm (Melissa officinalis L) and Moldavian dragonhead (Dracocephalum moldavica) belong to the Lamiaceae family. The plants due to its medicinal compounds, particularly essential oils; are very important for cultivation and quality improvement. The aim of this study was to investigate the effect of Nano-Fe fertilizer rates on seed germination indices. The experiment was carried out based on factorial complete randomized design with two factors and three replicates. The factors were Nano-Fe with five levels and genetics of the plants with three levels. The results showed that different levels of Nano-Fe had high significant effect on germination rate, mean of germination day and root to shoot ratio among genetic accessions. The interaction was significant only in terms of root to shoot ratio. The Nano-Fe fertilizer significantly improved the root length. The highest germination rate was obtained in treatment of 8 mg/L and M. officinalis genus. The highest root to shoot ratio was achieved in 4 mg/L of Nano-Fe fertilizer and M. officinalis genus. According to the electrophoresis results of nine types of protein band of the molecular weight between 10 to 150 kDa among the genetic masses, three proteins were polymorphism. The increase of Nano-Fe fertilizer led to the small increase of total protein and 16 mg/L treatment had the highest protein content, while, no alteration was discovered in the protein quality (protein band type). Moreover, it was found that the mass of M. officinalis had a bit higher protein content in comparison to two D. moldavica ones.