The effect of accelerated aging on seed germination traits produced from treated mother plant in three bean cultivars

Seed senescence is one of the major problems in the production of crops. In order to evaluate the effect of accelerated aging on germination and other seed germination components produced from the treated mother plant in three bean cultivars, a factorial experiment was conducted in a randomized complete block design with three replications at the research laboratory of Faculty of Agriculture, Shiraz University. The factors were including pinto bean varieties at 3 levels includes E9, E10, and Khomain, accelerated aging at two levels includes accelerated aging and control, and the third factor was seeds produced from treated mother plant seed in the farm at 7 levels includes: polyethylene glycol (PEG) -5 bar at 6 hours, , potassium chloride (KCl) -20 mM at 6 hours, calcium chloride (CaCl2) - 15 mM at 3 hours, sodium chloride (NaCl) -20 mM at 6 hours, salicylic acid (C7H6O3) 500 mg per liter at 12 hours, distilled water at 12 hours and without pretreatment (Control). The percentage and rate of germination, seedling dry weight, weight and length vigor index and 9th day germination energy were measured. The result showed that the interaction between accelerated aging, pretreatment and variety on germination traits including germination percentage, seedling dry weight, weight and length vigor index and germination energy of the 9th day at 1 % level, interaction between accelerated aging and pretreatment on germination rate at 1 % level and interaction between pretreatment and variety on germination rate and coefficients of allometric at 5 % level were significant. Seed germination traits at accelerated ageing treatment was lower than control. The maximum germination percentage and germination energy of the 9th day was for sodium chloride at 6 hours and E10 variety, maximum seedling dry weight, germination rate and weight and length vigor index in calcium chloride (CaCl2) at 3 hours treatment and E10variety were detected. The result showed that according to the variety and selection of appropriate pretreatment of mother plant, it is possible to improve storage and have seeds with higher power and less deterioration.Seed senescence is one of the major problems in the production of crops. In order to evaluate the effect of accelerated aging on germination and other seed germination components produced from the treated mother plant in three bean cultivars, a factorial experiment was conducted in a randomized complete block design with three replications at the research laboratory of Faculty of Agriculture, Shiraz University. The factors were including pinto bean varieties at 3 levels includes E9, E10, and Khomain, accelerated aging at two levels includes accelerated aging and control, and the third factor was seeds produced from treated mother plant seed in the farm at 7 levels includes: polyethylene glycol (PEG) -5 bar at 6 hours, , potassium chloride (KCl) -20 mM at 6 hours, calcium chloride (CaCl2) - 15 mM at 3 hours, sodium chloride (NaCl) -20 mM at 6 hours, salicylic acid (C7H6O3) 500 mg per liter at 12 hours, distilled water at 12 hours and without pretreatment (Control). The percentage and rate of germination, seedling dry weight, weight and length vigor index and 9th day germination energy were measured. The result showed that the interaction between accelerated aging, pretreatment and variety on germination traits including germination percentage, seedling dry weight, weight and length vigor index and germination energy of the 9th day at 1 % level, interaction between accelerated aging and pretreatment on germination rate at 1 % level and interaction between pretreatment and variety on germination rate and coefficients of allometric at 5 % level were significant. Seed germination traits at accelerated ageing treatment was lower than control. The maximum germination percentage and germination energy of the 9th day was for sodium chloride at 6 hours and E10 variety, maximum seedling dry weight, germination rate and weight and length vigor index in calcium chloride (CaCl2) at 3 hours treatment and E10variety were detected. The result showed that according to the variety and selection of appropriate pretreatment of mother plant, it is possible to improve storage and have seeds with higher power and less deterioration.