Effects of Nano-Zinc oxide and plant growth promoting rhizobacteria on zinc and protein content, phosphatase activity and related traits to grain growth of Triticale

In order to study of the effects ofnano-zinc oxide andseed inoculation with plant growth promoting rhizobacteria on phosphatase activity, zinc and protein content and related traits to grain growth of Triticale, a factorial experiment was conducted based on a randomized complete block design with three replications in research greenhouse of faculty of Agriculture Sciences, University of Mohaghegh Ardabili in 2013. Factors were seed inoculation with plant growth promoting rhizobacteria in four levels (without inoculation as control, seed inoculation with Azotobacter chrocoococum strain 5, Azosprillium lipoferum strain OF and Psedomunas putida strain 9) and foliar application of nano-zinc oxide at five levels (0 as control,0.25, 0.5, 0.75 and 1 g.lit-1). Means comparisons showed that maximum of phosphatase activity (1.35 nmol.min-1, zinc (31.4 mg.kg-1 and protein (134.2 g.kg-1) content, rate (0.10017 g.day-1) and grain filling period (40.74 days)were obtained at application of  1 g.lit-1 Nano-Zinc oxide and seed inoculation with Azosprillium and minimum of them (0.46 nmol.min-1, 23.1 mg.kg-1  ,108.6 g.kg-1, 0.0012 g.day-1 and 24.12 days respectively) were recorded at no application of nano-zinc oxide and without of seed inoculation with PGPR. Therefore, it seems that in order to improve seed quality, increasing of grain filling period, zinc and protein content, it can be suggested that be applied 1 g.lit-1 Nano-Zinc oxide ×seed inoculation with Azosprillium.