Improving the Viability of Microspores in Rosa hybrida cv. Apollo for Haploid Induction

Production of haploid plants in ornamentals is one of the breeding methods which have been restricted to only a few species. In order to optimize haploid culture in Rosa, the effects of two stress elements (Colchicine and 2,4-D) on microspore viability of R. hybrida cv. Apollo were studied. Two isolation techniques (pestle and magnet bar), three isolation media (NLN, AB, B), five induction media (NLN, A2, AT3 with 90 g.L-1maltose, AT3 with 180 g.L-1 maltose, AT3 with 90 g.L-1 glucose) were also compared. The results showed that, the best isolation technique, isolation medium and induction medium were to use magnet-bar, B medium (medium without any nutrients) and AT3 medium containing 90 g.L-1 maltose respectively. These results provided useful information on microspore isolation and survival for further studies in haploid induction in Rosa.