The effect of application of bioactive packing cover on the basis of natural preservative (chitosan / natamycin) and microalgae, Spirolina platensis and Chlorella vulgaris on Quality Characteristics and Beef Shelf Life

The aim of this study was to investigate the effects of antimicrobial coating based on chitosan, and natamycin, Chlorella vulgaris, Spirolina platensis for increasing the survival rate of beef. For this purpose, the values of zero, 1, 1.5 and 2 percent of the compounds were used during the days of zero, 7, 14 and 28 days storage in the packaging. Physicochemical meat tests including pH, ash percentage, protein, volatile nitrogen, index of thiobarbituric acid, texture index (Hardness, elasticity, cohesively, consistency, chewiness), total microbial population, sensory evaluation (crispiness, taste, wateriness, overall acceptance) in a completely randomized design with three repetitions at a significant level of 95% confidence has been checked. The results of the experiments were analyzed by using Minitab 17.2 software. The results showed that there were significant differences between the calf meat treatment Physicochemical and sensory with the testator treatment and increasing the use of chitosan, algae and also kanamycin, increase of microbial index rate, volatile nitrogen index (TVBN), thiobarbituric index (TB) during storage time for 28 days will be happened by less intensity. But the percentage of ash index did not show significant differences (p<0.05). The use of algae in packaging formulations increases by up to 1%, increased hardness, decreased elasticity, chewiness and cohesiveness, as well as gumminess. With the increasing in the use of chitosan and the prevention of microbial and antioxidant damage as well as the use of algae due to its antioxidant properties, the rheological properties of the tissue as well as sensory evaluation were significantly less than the control treatment. Finally, taking into account the Physicochemical properties, the sensory packaging coverage by using 1% platinaceous algae, Chlorella vulgaris1% ,Spirolina platensis,1% chitosan and 1% natamycin was used as the best composition in order to cover packaging.