Association of haptoglobin and natural resistance-associated macrophage protein 1 alleles with heme-consuming periodontal pathogens in chronic periodontitis and peri-implantitis: A pilot study

This study aimed to assess the association of haptoglobin (HP) and natural resistance-associated macrophage protein 1 (NRAMP1) alleles with the presence of heme-consuming periodontal pathogens in a group of Iranian patients with chronic periodontitis and peri-implantitis.

This cross-sectional study evaluated 69 eligible chronic periodontitis and peri-implantitis patients selected from Shahid Beheshti Dental School. Periodontally diseased individuals had to have at least 3 teeth with clinical attachment loss ≥ 3 mm/probing pocket depth (PPD) ≥ 3 mm in at least two quadrants. Peri-implant PPD of at least one site had to be ≥ 5 mm with or without suppuration/bleeding on probing. Plaque index > 20% and radiographic crestal bone loss had to be present in at least one site around the implant. The paper point method was used for sampling from the deepest periodontal/peri-implant pocket of each tooth or implant for the DNA checkerboard hybridization technique. Statistical analyses were performed with PASW Statistics 18.0. The variables were presented as absolute and relative frequencies (%).

Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) score 1-2 was 5.8 times more frequent in HP 2, rs1723540 G and rs2276631 G alleles. Porphyromonas gingivalis (P. gingivalis) score 1-2 was 4.8 times more common in people carrying HP 2, rs1723540 G and rs2276631 G alleles compared with HP 1, rs1723540 A and rs2276631 A alleles.

Within the limitations of this study, it seems that there is a relationship between HP and NRAMP1 allele frequencies and presence of heme-consuming periodontal pathogens in Iranian patients with chronic periodontitis and peri-implantitis.