The Effect of Different Combinations of Hormonal Treatments on Breaking Seed Dormancy in Different Ecotypes of Black Zira (Bunium persicum)

To study the effect of different hormone combinations on breaking dormancy and germination of Black Zira, 27 treatments consisted of three hormone, gibberellic acid (80, 100, and 120 μm), Thidiazuron (4, 6 and 8 μm) and benzyl adenine (5, 10 and 15 μm). Factorial experimental with three replications in a randomized complete block design was carried out. Seedling length, seedling fresh weight, seedling dry weight and the total percentage of germination were measured and seedling vigor index, fresh weight index, and dry weight index were calculated. Analysis of variance showed the significant differences between the effect of ecotype × combination × Hormone level for fresh weight, total percentage of germination and vigor index. Comparison of means for ecotype × combination × level effect showed the total percentage of germination and vigor index were highest for sixth level of GA × BA combination in Hormozgan ecotype and fresh weight was highest for fifth level of TDZ × BA combination in Semnan ecotype. Mean Comparison of ecotype effects showed that the dry weight index was the highest in Semnan ecotype and the Mean Comparison of level effects showed that the dry weight index was highest in fourth levels (6 μm TDZ and 5 μm BA and 100 μm GA). The result showed that hormones levels and combinations were suitable for seed germination in this experiment. Hormozgan ecotype had the best germination at GA × BA combination.