Distribution, morpholy and Phylogenetic Study of Hypoderma bovis by PCR and CO1 gene sequence analysisin in three climate regions in Iran

Hypoderma bovis (Diptera: Oestridae) is an important parasite in cattle that is pathogenic and cause serious economic losses and reduce livestock products, including milk, meat and hide quality.In this study, samples from slaughtered cattle were collected from three climate of Iran. Extraction of DNA and PCR test was performed on the samples, then the purified PCR product was sequenced. Sequence response was reviewed and corrected. Finally, the distribution of this species was investigated according to morphological characteristics in three climate zones of Iran. The CO1 gene of third stage larvae of H. bovis was investigated in the collected larvae. Data showed interspecies correlation. Based on morphological observations, from total of 416 third stage larvae obtained from slaughtered cattle in three regions, 268 (64.4%) were H. bovis. Based on phylogenetic analysis of the present isolates, these isolates are located in a cluster with a number of Hypoderma species of studied in other countries, while some other species are isolated in the other cluster. The COl region seems to be very variable among the miyasis caused flies. The findings also suggest that CO1 gene can be used as an appropriate molecular marker for identification and diagnosis of Hypoderma species.