The Study of effective factors in callus induction, somatic vegetative and regeneration in Paulownia ShanTong

The present study was carried out to investigate callus induction, vegetative embryogenesis and indirect regeneration of Paulownia shangtong. This experiment was conducted as a factorial experiment in a completely randomized design with five replications. The studied factors were explants and different compositions of the Murashik-Skogh medium. The interaction effect of explant and medium was significant for callus production rate, callus production percentage, callus area and vegetative embryogenesis percentage. The results of the mean comparison showed that the highest regeneration rate of vegetative embryos was obtained with petiole explant and medium of½&A/4 MS + 0.3 mg/l + 2mg/l KIN + 4 mg/l BAP + 0.30 mg/l. To test the root induction of regenerated seedlings a factorial experiment was conducted based on a randomized complete block design with five replications. The factors were different mediums (MS medium and 1/2MS medium) in combination with hormones of IBA, NAA and IAA. The highest percentage of rooting was observed in ½MS supplemented with 1.5 mg / l IBA. The regeneration of vegetative embryos in MS base medium was less than other mediums.