Effect of Copper Sulfate Replacement with Copper Nano Oxide in Diet on Growth performance and Enzymatic Activity and Tissue Pathology of Goldfish Liver (Carassius auratus)
The purpose of this study was to investigate the effect of replacement of copper sulfate with nano oxidase in diet on growth and survival, enzymatic activity and pathology of liver tissue of goldfish in 2018.
Experimental treatments consisted of 5 control treatments, 3 mg / kg copper sulfate; 3 mg / kg nano-oxide, 5 mg / kg nano-oxide and 10 mg / kg nano-oxide, and fish were given 4% by weight twice a day for 60 days. The body was fed manually. At the end of the trial period, growth and survival indices , liver enzymes activity (alkaline phosphatase, aspartate transaminase, alanine, Aminotransferase) as well as liver tissue pathology were evaluated.
Based on the results, using copper nanoparticles at different levels of 3, 5 and 10 mg / kg diet instead of copper sulfate in the diet can improve the growth performance and survival of goldfish. Copper sulfate also has more negative effects on liver tissue than nanoparticles, as well as alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase enzymes; therefore, the replacement of copper sulfate with copper nanoparticles in the diet seems to reduce the effects of water. On the other hand, histopathological results of liver tissue treatment with 5 and 10 mg Cu / kg diet showed that Cu nanoparticles cause tissue lesions and deleterious effects (albeit less than copper sulfate).
Copper sulfate with copper nanoparticles at levels of 3 mg / kg diet To be carried out.
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