Evaluation of ESR and IGFBP1 genes of ovarian tissue of endometriosis model mice after a period of regular exercise and vitamin E intake

The aim of this study was to investigate the ESR and IGFBP1gene expression changes in endometriosis model rats following a period of aerobic exercise and vitamin E intake.

In this experimental study, 25 adult Wistar rats were randomly divided into 5 groups of healthy control, endometriosis, endometriosis+ exercise, endometriosis+ vitamin E, endometriosis+ vitamin E+ exercise after creating endometriosis model. Vitamin intake was 200 kg/mg per rat body weight and swimming program was 8 weeks, five days a week for 30 minutes each day. One-way ANOVA test were used for data analysis.

The results showed that the highest expression of ESR and IGFBP1 gene was observed in endometriosis+ exercise+ vitamin E group and the lowest levels in endometriosis group. Intergroup comparisons showed that there was a significant difference between endometriosis and endometriosis+exercise, endometriosis+vitamin E and endometriosis+exercise+vitamin E groups in ESR gene expression (p=0.0016, p=0.026 , p=0.0335, respectively). There was also a significant difference between endometriosis group with endometriosis+exercise and endometriosis+exercise+vitamin E and also between endometriosis+exercise+ vitamin E groups with endometriosis+exercise and endometriosis+vitamin E groups in IGFBP1 gene expression (p=0.0019, p=0.0446, p=0.002, p=0.0222, respectively).

In general, the results of this study indicated that alteration of key molecules or signaling pathways and gene expression in endometriosis can improve the level of this disease and regular aerobic exercise as well as concomitant use of Vitamin E can help prevent and improve this disease.