The Effect of Mycotoxin Binder Toxeat® on The Expression of INFγ and INFα Genes and Serum Levels of IL2 and IL6 in Broiler Chickens Fed with Aflatoxin

The aim of this study was to investigate the effect of Mycotoxin Binder Toxeat® in reducing the negative effects of aflatoxin on the expression of INFγ and INFα genes and serum levels of IL2 and IL6 in broiler chicks. 300 oneday-old (Cobb 500) broiler chicks were used. They were randomly assigned to six treatments, 5 replicates and 10 birds in each unit. Experimental treatments included: Base diet (T1), Base diet+1 mg/kg of aflatoxin (T2), T2+1 g/kg Toxeat® (T3), T2+2 g/kg Toxeat® (T4), T2+3 g/kg Toxeat® (T5), T2+1 g/kg Toxeat® without inorganic section (sodium-calcium hydrated aluminosilicate hydrate) (T6). On day 42 livers were collected (3 per treatment) to evaluate changes in the expression of INFγ and INFα genes after reverse transcription of RNA extracted from liver tissue were taken. The results of this study showed that the expression of INFγ and INFα genes were different in all experimental treatments, so that the expression of INFγ gene increased significantly in treatment 3 compared to other treatments (P <0.05). Also, expression of INFα gene  in Myxotoxin Binder Toxeat® receiving treatments increased significantly compared with the control group (P <0.05). In the study of IL6 serum concentration showed a significant difference between treatments (P <0.05) but there was no significant difference in IL2 serum concentration between treatments (P> 0.05). Generally, the results of the study showed that diet supplements with Mycotoxin Binder Toxeat® could be effective in controlling the undesirable effects of Aflatoxin, it has an effect on gene expression and production and secretion of cytokines resulting in a reduction in the mortality of broiler chicks by improving the immune response