The effect of eccentric resistance exercise and blood flow occlusion on STAT3 gene expression and MyF5 affecting the activation of satellite cells on non-athletes

The aim of the present study, the effects of resistance training on gene expression and blocking blood flow signal transfer agent and activator of transcription (STAT3) and muscle-forming factor 5 (Myf5) is non-active young people.

The study population consisted of 20 active non-athletic young men aged 25 ± 5 years who were randomly assigned to two groups of ten eccentric resistance exercise with and without blood flow restriction (BFR). Using isokinetic, eccentric resistance exercise protocol (ECC RET) containing about 70 repetitions eccentric resistance exercise on knee extensor muscles was performed. For occlusion of blood flow using the air pressure gauge with width of 5 cm. The STAT3 and MyF5 measured by immunohistochemistry.

The expression of STAT3 gene in the samples compared to pre-test and post-test BFR group was 3.846 times the amount (p = 0.001) showed a significant difference, and gene expression in samples BFR MyF5 in the group after the test the pre-test was 3.479 times the amount (p = 0.005) showed a significant difference.

The use of eccentric resistance exercise combined with occlusion of blood flow through the increased expression of STAT3 gene and MyF5 can help maintain and improve muscle mass.