The effect of vigorous continuous and interval exercise training along with resveratrol on SIRT3 and OGG1 proteins in the liver tissue of male Wistar rats

Perpose: Sirtuins-3 (SIRT3) is a mitochondrial deacetylase and through acetylation of Oxoguanine Glycosylase 1(OGG1) protein caused repair mitochondrial DNA damage and prevents cell apoptosis. The purpose of this study was to evaluate the effect of vigorous continuous and interval exercise training along with resveratrol on SIRT3 and OGG1 proteins in the liver tissue of male Wistar rats.

Forty eight male Albino Wistar rats divided into six equal groups including sedentary control, resveratrol, interval exercise, interval exercise + resveratrol, continuous exercise and continuous exercise+ resveratrol. Continuous and interval exercise performed for 6 weeks, six days per week, up to 80% and 95 -100% VO2max respectively on a rat treadmill. Resveratrol users received daily supplements as 50 mg/kg of body weight. After intervention, the animals were anesthetized and their liver samples were taken for analysis.

After 6 weeks of vigorous exercise training and resveratrol consumption, OGG1 level was significantly increased in continuous exercise (p=0.0001) and continuous exercise + resveratrol (p = 0.0001) groups. In addition, the level of liver SIRT3 increased in the resveratrol (p = 0.01), interval exercise (p = 0.04), interval + resveratrol (p = 0.04), continuous exercise (p = 0.001) and continuous exercise + resveratrol (p = 0.001) groups; so that the induced elevation in the dependent variables of continuous + resveratrol group was significantly higher than other groups (p = 0.001).

Compare to the interval exercise, performing of continuous exercise along with resveratrol consumption can induce more improvement in the liver mitochondrial function and biogenesis.