Experimental Study of the Histological Method for Quantitative Detection of Meat in Kabab and Cooked Sausage Model

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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
BACKGROUND

Regarding the economic values of meat, adulteration in meat products is probable.

OBJECTIVES

This study was carried out to evaluate the histological method for the quantitative detection of meat in Kabab Loghme and cooked sausage model.

METHODS

Five Kabab samples (containing 70 % meat) and cooked sausage (30, 50, 70 and 90% meat), were prepared. Then, each sample was divided into three parts and one piece was taken from each part and fixed in 10% neutral-buffered formalin. The samples were routinely processed for light microscopy and embedded in paraffin. The paraffin-embedded blocks were cut into 6 μm sections and stained using hematoxylin and eosin (H & E) for histological study.

RESULTS

The histometrical analysis indicated that the estimated percentages for the added meat in kabab did not show significant difference with the real related percentages. On the other hand, the amount of meat was difficult to estimate especially in cooked sausage.

CONCLUSIONS

The findings of the present research suggest the histological technique as a complementary method for quantitative evaluations of meat in raw meat products. However, the quantitative evaluation of meat in raw meat products was more convenient than in processed ones.

Language:
Persian
Published:
Journal of Veterinary Research, Volume:75 Issue: 3, 2020
Pages:
366 to 370
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