Effect of Combined and Isometric Exercise Training on SIRT1 Level in Elderly Women with Osteopenia

SIRT1 protein has been investigated as a key factor in many biological functions, such as longevity, oxidative stress, and bone mass in interaction with physical activity. The purpose of the present study was to compare the effect of combined and isometric exercise training on plasma level of SIRT1 in elderly women with osteopenia.

For this purpose, 45 women, aged 50-60 years, with osteopenia who were eligible for the present study, were selected and randomly divided into three groups of 15, each consisting of two exercise groups (Combined group and Isometric group) and one control group. After grouping the participants' initial blood sampling, Co group was trained 45-90 min per session (stretch, strength, endurance) and Iso group was trained 20-40 min (set, frequency, contraction time), three sessions per week, for 12 weeks. Data were analyzed using SPSS, version 24, running using paired t-test, One-way ANOVA, and Tukey post hoc test with the significance level set at 0.05.   

The ELISA results showed that both exercise methods had significant effects on SIRT1 compared to baseline (p=0/01). There was a significant difference between the two methods of training with control group on SIRT 1 level in the elderly women with osteopenia (p=0/001), but the difference between the two was not statistically significant (p=0/979). 

It seems that elevated SIRT1 level in aged people is compensatory mechanism of stress oxidative system and decreased activity in growth factor such as IGF1 in old age. It can be concluded that combined and isometric exercise could have positive effects on SIRT1 level in elderly women with osteopenia.